Research Associate I – Wolfe Lab

Under the direction of the Principal Investigator (PI) or designee, the Research Associate I provides operational support to the laboratory. The Research Associate I will perform research experiments as defined by the PI.

Perform laboratory experiments and may be responsible for a single research project
• Utilize all software relevant to experiment and equipment used
• Interpret data, form conclusions and decide on next steps under the direction of the PI or designee
• Maintain accurate records, may modify protocols with the approval of the PI or designee
• Review literature, publications and grants relevant to assigned projects
• Record results according to established lab, sponsor & university protocol
• Assist with performing data analysis and/or mathematical computations
• Maintain a detailed lab notebook according to lab practice
• Present findings at lab/group meetings
• Monitor laboratory supply levels and order as noted
• Prepare and label buffers, media, solutions, etc.
• Assist with and comply with all safety regulation and infection control standards
• Train on IACUC, IRB, EHS, Radiation safety protocols
• Comply with all applicable state, federal university, and sponsor policies and agreements
• Provide support with identifying problems and challenges, propose resolutions, implement appropriate solutions
• Provide support with drafting standard operation procedures in compliance to EHS regulation specific for the lab
• Perform other duties as required

• Bachelor’s degree in Biological or Natural Science, or equivalent experience
• Basic understanding of lab safety and protocols
• Knowledge of Microsoft Office products
• Ability to analyze data in a quantitative and objective manner.
• Ability to work in a team environment

The focus of the Wolfe Laboratory is the development of genome editing tools, such as base editors and prime editors, to employ as new classes of therapeutics. The successful candidate would be a part of an interdisciplinary team of laboratory scientists, clinicians, and computational biologists that are working on therapeutics for a variety of neurological and neuromuscular disorders, as well as cancer. We are looking for a candidate that would be a key player in developing and characterizing novel genome editing systems that could serve as new therapeutics – with these reagents potentially being used in clinical trials.

Experience Required:

1. Basic molecular biology techniques (PCR, cloning, gel electrophoresis, plasmid purification).
2. Mammalian Cell culture experience.

Experience Desired (not required):

1. Genome editing
2. Illumina Sequencing library preparation
3. Experience using mouse disease models

Major Responsibilities (in addition to the standard set):

1. Participate in the development and characterization of genome editing systems tailored to correct gene mutations common in certain patient populations or kill cancer cells.
2. Participate in the Application of these genome editing systems in relevant mouse disease models.
3. Organize ordering and stocking of supplies and reagents for the laboratory.