Job title:
Research Scientist – Vector Development
Company
Cranleigh Recruitment
Job description
An exciting opportunity is available for an R&D Scientist with expertise in Vector Development to join a growing start-up. With state-of-the-art facilities and a robust pipeline of upcoming projects, this role offers the chance to contribute to innovative research and development.Responsibilities:The purpose of this role is to develop mammalian plasmid constructs for optimal expression of antibody therapeutics to be used in mammalian bioprocesses. As a Scientist you will be part of an innovative multidisciplinary team that will develop the next generation of mammalian expression systems & cells lines to enable highly productive pharmaceutical bioprocesses and, ultimately, more cost effective Immunotherapeutics.
- Design and screen mammalian gene expression plasmids for high and stable expression of therapeutic antibodies including bi-/tri-specific antibodies
- Design and optimise CRISPR knock-out and ORF libraries to be used in high throughput functional genomics pipelines
- Design, plan, and execute complex R&D experiments ensuring that deliverables are successfully met within timelines
- Document research findings clearly in a lab book
- Communicate regularly in group meetings and prepare and deliver presentations to project team and internal stakeholders
- Along with team members, provide routine laboratory maintenance, including participating in equipment upkeep, cleaning rotas and laboratory consumables stock management
- Shared responsibility for out-of-hours cell culture maintenance.
- Ensure that laboratory health, safety and quality procedures are followed
- Select, follow, and adapt experimental protocols and generate laboratory SOPs
- Support the preparation of laboratory risk assessments and maintenance of health and safety protocols
- Maintain the confidentiality of the project and protocols
- Keep abreast of current technologies and relevant scientific literature to help identify opportunity for improvements and reduction of costs
Experience RequiredEssential Experience:
- BSc/MSc in Biochemistry, Molecular Biology or related field
- BSc/MSc with 3+ years of industry experience or PhD with 1-2 years’ experience
- Extensive experience of designing, preparing and screening mammalian gene expression plasmids within the context of mammalian cell line development
- Experience of designing CRISPR knock-out and ORF libraries for high-throughput functional genomics screens
- Experience of working safely with 3rd generation lentiviral plasmid systems within a CAT2.0 environment
- Experience of designing mammalian gene expression constructs for Immunotherapeutics molecules eg. antibodies
- Experience of working with bioprocess relevant cell lines eg. CHO or HEK293
- Familiarity of mammalian cell line development including generation of GM/GE cell lines, single cell cloning and clonal screening
- Experience of mammalian cell culture and related techniques including passaging, banking, transfection, nucleofection & genotyping
- Skilled in molecular biology techniques including PCR, qRT-PCR, Western Blot, ELISA, plasmid preparation, plasmid cloning, site directed mutagenesis & FACS
- Experience of working with SnapGene or related software
- Skilled in experimental design and troubleshooting
- Ability to work independently and as part of team
- Good numeracy, computer and communication skills
- Experience of writing SOPs and risk assessment
Desirable Experience:
- Application of Design of Experiments (DOE) to rationalise experiment design
- Familiarity of working with large datasets including transcriptomic / proteomic datasets
- Experience with automation and high-throughput technologies applied to mammalian cell line generation eg. Beacon, 96 deep well plate model systems & Ambr15
£40k – £55k + Benefits
Expected salary
£40000 – 55000 per year
Location
Oxfordshire
Job date
Fri, 22 Nov 2024 23:24:23 GMT
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